OpenAccessBelV2, OpenAccess, Analysis, bibRecord, 000060

Adipose Mesenchymal Stromal Cell Function Is Not Affected by Methotrexate and Azathioprine

Identifieur interne : 000060 ( OpenAccess/Analysis ); précédent : 000059; suivant : 000061

Adipose Mesenchymal Stromal Cell Function Is Not Affected by Methotrexate and Azathioprine

Auteurs : Pablo Manche O-Corvo ; Marcella Franquesa ; Olga De La Rosa ; Cristina Ramírez ; Leyre García-Benzaquén ; Vanessa Fernández ; Ram N Menta ; Aitor Beraza ; Wilfried Dalemans ; Martin J. Hoogduijn ; Eleuterio Lombardo

Source :

BioResearch Open Access [ 2164-7844 ] ; 2013.

RBID : PMC:3869447

Abstract

Given their capacity to modulate the immune response, adipose mesenchymal stem or stromal cells (ASCs) have been used as therapeutic tools to treat chronic inflammatory and autoimmune diseases both in preclinical and clinical studies. Patients enrolled in such clinical trials are often concomitantly treated with immunomodulatory drugs such as methotrexate (MTX) or azathioprine (AZA). Therefore it is necessary to investigate the possible impact of these drugs on ASC function to learn if there are any interactions that would affect the therapeutic effects of either component and thus the clinical outcome of the trials. ASCs were cultured in the absence or presence of MTX or AZA and the effects on viability, proliferation, immunomodulatory properties, and immunogenic features were studied in vitro. The drugs did not affect the viability and proliferative capacity of ASCs. When the drugs and the ASCs were concomitantly used to inhibit lymphocyte proliferation, no synergistic or antagonizing inhibitory effects were found. MTX and AZA did not impair the capacity of ASCs to induce the generation of regulatory T cells in vitro. These data confirm that the immunomodulating features of ASCs are fully functional after exposure to these drugs. Interestingly, whereas MTX did not affect the capacity of natural killer (NK) cells to lyse allogeneic ASCs in vitro, AZA protected allogeneic ASCs from NK cell lysis.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3869447

DOI: 10.1089/biores.2013.0040
PubMed: 24380053
PubMed Central: 3869447

Affiliations:

Links to Exploration step

PMC:3869447

Le document en format XML

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<front><div type="abstract" xml:lang="en"><title>Abstract</title>
<p>Given their capacity to modulate the immune response, adipose mesenchymal stem or stromal cells (ASCs) have been used as therapeutic tools to treat chronic inflammatory and autoimmune diseases both in preclinical and clinical studies. Patients enrolled in such clinical trials are often concomitantly treated with immunomodulatory drugs such as methotrexate (MTX) or azathioprine (AZA). Therefore it is necessary to investigate the possible impact of these drugs on ASC function to learn if there are any interactions that would affect the therapeutic effects of either component and thus the clinical outcome of the trials. ASCs were cultured in the absence or presence of MTX or AZA and the effects on viability, proliferation, immunomodulatory properties, and immunogenic features were studied <italic>in vitro</italic>
. The drugs did not affect the viability and proliferative capacity of ASCs. When the drugs and the ASCs were concomitantly used to inhibit lymphocyte proliferation, no synergistic or antagonizing inhibitory effects were found. MTX and AZA did not impair the capacity of ASCs to induce the generation of regulatory T cells <italic>in vitro</italic>
. These data confirm that the immunomodulating features of ASCs are fully functional after exposure to these drugs. Interestingly, whereas MTX did not affect the capacity of natural killer (NK) cells to lyse allogeneic ASCs <italic>in vitro</italic>
, AZA protected allogeneic ASCs from NK cell lysis.</p>
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Adipose Mesenchymal Stromal Cell Function Is Not Affected by Methotrexate and Azathioprine

Adipose Mesenchymal Stromal Cell Function Is Not Affected by Methotrexate and Azathioprine

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Abstract

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